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WebDec 9, 2024 · Cells were grown in Marine Broth (MB) medium (Difco 2216, BD Biosciences, San Jose, USA) at 25 °C with shaking at 200 rpm and harvested during late logarithmic growth phase (OD 600nm = 0.5–0.8). Aliquots containing 20% glycerol were then stored at −80 °C. In order to perform further analyses on living cells, stock cultures were slowly ...
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