Web%0 Journal %B Nature Protocols %D 2010 %T Fluorescent labeling of tetracysteine-tagged proteins in intact cells %A Hoffmann, C. %A Gaietta, G. WebFirst, we confirm the resulting enhanced FRET efficiency on our nanocoatings for the inactive M1 muscarinic acetylcholine receptor (mAChR) labeled with a FRET pair of cyan …
β-arrestin–based biosensors: Tools to explore structural
WebAug 27, 2024 · 因此,他们进行了荧光素砷化发夹结合剂荧光共振能量转移实验 ( fluorescein arsenical hairpin binder ( FlAsH ) –based fluorescence resonance energy transfer ( FRET ) assay , FlAsH-based FRET )(这一方法可最小化对 GPCR 活性检测的干扰 【 11 】 ) 来检测 Aβ 富集区的 Ghsr1ɑ 活性 。 WebJun 28, 2024 · The fluorescein arsenical hairpin binder (FlAsH) fluorescein tag contains the amino acid sequence of CCPGCC, within which the tetracysteine motif is labeled with FlAsH-ETD2 fluorescein (Fig. 1b ). Fig. 1 pOPHBL and pOPHFL bacterial expression vectors. ( a) A map of the pOPHBL plasmid. DNA sequences and their amino acid … mayfield ohio building department
Fluorescent labeling of tetracysteine-tagged proteins in
FlAsH-EDT2 is an organoarsenic compound with molecular formula C24H18As2O5S4. Its structure is based around a fluorescein core with two 1,3,2-dithiarsolane substituents. It is used in bioanalytical research as a fluorescent label for visualising proteins in living cells. FlAsH-EDT2 is an abbreviation for … See more FlAsH-EDT2 can be prepared in three steps from fluorescein (see figure). See more Many studies show that trivalent arsenic compounds bind to pairs of cysteine residues. This binding is responsible for the toxicity of many arsenic compounds. Binding is reversed … See more FlAsH-EDT2 enables less toxic and more specific fluorescent labeling that is membrane permeable. The modification of the fluorescein moiety also allows multicolor analysis. … See more FlAsH becomes fluorescent upon the binding of tetracysteine motif. It is excited at 508 nm and emits 528 nm, a green-yellow, of free fluorescein. The quantum yield is 0.49 for 250 nM FlAsH is bound to a model tetracysteine-containing peptide in a phosphate … See more WebAug 1, 2012 · fluorescein arsenical hairpin binder PLC phospholipase C PCR polymerase chain reaction PH plecktsrin homology HEK293 human embryonic kidney cells 293 HBSS Hanks' balanced salt solution PtdIns(4,5)P 2 phosphatidylinositol-4,5-bisphosphate. Received January 3, 2012. Accepted May 7, 2012. Webwe developed sensitive assays that use the fluorescein arsenical dye FlAsH (fluorescein arsenical hairpin binder) to detect solu-ble oligomers and mature fibrils formed from recombinant-syn protein containing an N-terminal bicysteine tag (C2- tia in PD, correlates with more widespread-syn). Using seed growth by monomer association (SeGMA) herters powder measure