Phospho-akt1/2/3 ser473 cellular kit

Author: heps

August undefined, 2024

WebPhospho-Akt (Ser473) Kit For use with human, mouse, rat, and non-human primate cell lysates and tissue lysates. This package insert must be read in its entirety before using … WebFeb 3, 2024 · Protein kinase B (AKT1) is hyper-activated in diverse human tumors. AKT1 is activated by phosphorylation at two key regulatory sites, Thr308 and Ser473. Active AKT1 phosphorylates many, perhaps hundreds, of downstream cellular targets in the cytosol and nucleus. AKT1 is well-known for phosphorylatin …

Phospho-EGFR (Tyr1068) cellular HTRF kit Cisbio - PerkinElmer

WebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an oncogene playing a key role in controlling apoptosis, cell proliferation, transcription, cell migration, and glucose metabolism. WebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an … notenpuree

Phosphorylated AKT1/2/3 (S473) LANCE Ultra …

WebThe Phospho-AKT (Ser473) kit is designed to robustly and efficiently monitor AKT phosphorylation on Ser473 across all isoforms, as a readout of Pi3K pathway activation. … LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells. WebPhospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues. It does not recognize Akt phosphorylated at other sites, nor does it recognize phosphorylated forms of related kinases such as PKC or … notenprompter

Drug-screening and genomic analyses of HER2-positive breast …

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kits, PerkinElmer

Web寻找 Abcam 抗体及试剂 - Abcam中文官网 WebMyricetin ameliorates bleomycin-induced pulmonary fibrosis in mice by inhibiting TGF-β signaling via targeting HSP90β how to set scheduled shutdown windows 10WebThe phospho-EGFR assay kit is designed for the cell-based quantitative detection of Tyr1068 phosphorylation on EGFR. Mutations that lead to an overexpression or hyperactivity of EGFR, also known as ErbB1 or HER1, are associated with a number of cancers, such as lung, colorectal, prostate, glioblastoma cancer, etc. notenoughtech server

"WebIn contrast to previous studies suggesting the impact of Ser473 and Ser824 phosphorylation on TRIM28 function, examined phospho-mutants and phospho-mimetics did not affect … " - Phospho-akt1/2/3 ser473 cellular kit

Phospho-akt1/2/3 ser473 cellular kit

Phospho-Akt (Ser473) Antibody Cell Signaling Technology

WebAkt1/2 kinase inhibitor indeed decreased pAkt in all four cell lines , while total Akt levels were not affected by the treatment. To determine whether induction of apoptosis caused the decreased cell viability induced by the Akt1/2 kinase inhibitor, we measured the activity of caspase 3/7 in the treated cells by Caspase-Glo® assay.

Did you know?

WebAKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration. There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3. AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation. WebAKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of …

WebAlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kits, PerkinElmer Supplier: Perkin Elmer Harmonized Code US-3822005090 The AlphaLISA® SureFire® Ultra™ p-AKT1/2/3 … WebPhospho-Akt (Ser473) Rabbit mAb has been coated on the microwells. After incubation with cell lysates, phospho-Akt (Ser473) protein is captured by the coated antibody. Following extensive washing, Akt1 Mouse Antibody is added to detect the captured phospho-Akt1 (Ser473) protein.

WebProduct Note This antibody is raised against human AKT1 phosphorylated at Ser473. Based on sequence homology, it is predicted to react with AKT2 and AKT3 when phosphorylated at the corresponding residues - AKT2 Ser474 and AKT3 Ser472. Predict Reactivity Zebrafish, Sheep, Bovine, Cat, Chicken, Rhesus Monkey (>80% identity) PROPERTIES TARGET WebPhospho-ERK assay kits are designed for the robust quantification of ERK modulation, phosphorylated on Thr202/Tyr204, for GPCR and RTK targeted screening as a MAPK …

WebPhospho-AKT1 (Ser473) Antibody (700392) in ICC/IF Immunofluorescent analysis of AKT (pS473) was done on 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature.

WebAlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kits, PerkinElmer Supplier: Perkin Elmer Harmonized Code US-3822005090 The AlphaLISA® SureFire® Ultra™ p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Order Now … how to set scoreboard in minecraftWebUp-regulation of O-GlcNAcylation increases Akt1 phosphorylation at Ser473. The 8305C cells were treated with 5 μM Thia-G or were transfected with 500 ng OGT plasmid, and then cell lysates were resolved by SDS-PAGE, and phospho-Akt1 (Ser473) or Akt1 were determined with specific antibodies (Figure 4A and B). An increase in Ser473 ... how to set screen bounds on pcWebApr 14, 2024 · Notably, when proline in the 14-3-3 binding motif was replaced with an alanine (P436A), the 14-3-3 recognition of SIK1 was profoundly attenuated (Supplementary Fig. S4G). Together, these data suggest that AKT-mediated SIK1 phosphorylation enhances SIK1 binding with 14-3-3, which in turn increases the rate of SIK1 transfer to the cytoplasm. notenprogramm windows 10WebThe Total SHP1 cellular assay monitors the expression level of SHP1, and can be used as a normalization assay with our phospho-SHP1 Y564 kit. Because these phospho and total SHP1 assays are compatible, the two kits can be used in parallel on the same lysates. Many cancer cells overexpress checkpoint inhibitor ligands such as PD-L1. notenrechner fos bosWebNov 25, 2015 · The ER also plays a critical role toward maintenance of cellular calcium homeostasis. ... and the following oligonucleotide primers were used for RT-PCR: Akt1 forward, 5′-CTGTCTCGAGAGCGTGTGTT-3′; Akt1 reverse, 5′-AACAGCTTCTCGTGGTCCTG-3′; Akt2 ... [rabbit polyclonal anti-phospho-Akt (Ser473) antibody (Cell Signaling Technology, … notenrechner jura bayernWebMMP1 Antibody SKU: A... how to set screen 1 and 2 on windows 10WebThe phospho-AKT1 (Ser473) kit is designed for robust quantification of AKT1 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), Akt is an oncogene … notenprogramm download